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Runner up to Hillcrest location for "gayest clientele"
It doesn't have the constant presence of overtly gay exercisers, but Mission Valley is the second-gayest 24 Hour Fitness location in town, with Horton Plaza, Stadium, and Point Loma runners up. Mission Valley 24 Hour Fitness is much more spacious and has more equipment than the Hillcrest fishbowl, though also more straight people (but many are very fit and may help motivate your workout nonetheless). Some gays who don't like the high cruise factor at the Hillcrest location come to Mission Valley to get away from that, which explains the homo-population here. Easier to park than Hillcrest as well, though that's true of all other locations.
Large Gym with Lots of Parking
While it's not the hip-happening gym that the Hillcrest 24-Hour is, it's about triple the size, with many more equipment choices, and tons of parking. It's still very gay-friendly and has quite a selection of eye candy to boot.